Fig. 6. Cbl-b and c-Cbl ubiquitinate different lysine sites of STAT5.
The ubiquitination of STAT5a (A) and STAT5b (B) by Cbl-b and c-Cbl in HEK293T cells. C Immunoprecipitation analysis of ubiquitination of STAT5b by Cbl-b in HEK293T cells transfected with Flag-STAT5b (WT or mutant). D Immunoprecipitation analysis of linear ubiquitination of STAT5b by c-Cbl in HEK293T cells transfected with Flag-STAT5b (WT or mutant). E Immunoprecipitation analysis of linear ubiquitination of STAT5a by Cbl-b in HEK293T cells transfected with Flag-STAT5a (WT or mutant). F Immunoprecipitation analysis of linear ubiquitination of STAT5a by c-Cbl in HEK293T cells transfected with Flag-STAT5a (WT or mutant). Data are pooled from three independent experiments.