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. 2022 Mar 30;8:142. doi: 10.1038/s41420-022-00953-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — The ubiquitination of STAT5a (A) and STAT5b (B) by Cbl-b and c-Cbl in HEK293T cells. C Immunoprecipitation analysis of ubiquitination of STAT5b by Cbl-b in HEK293T cells transfected with Flag-STAT5b (WT or mutant). D Immunoprecipitation analysis of linear ubiquitination of STAT5b by c-Cbl in HEK293T cells transfected with Flag-STAT5b (WT or mutant). E Immunoprecipitation analysis of linear ubiquitination of STAT5a by Cbl-b in HEK293T cells transfected with Flag-STAT5a (WT or mutant). F Immunoprecipitation analysis of linear ubiquitination of STAT5a by c-Cbl in HEK293T cells transfected with Flag-STAT5a (WT or mutant). Data are pooled from three independent experiments.