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. 2022 Mar 30;8:142. doi: 10.1038/s41420-022-00953-2

Fig. 6. Cbl-b and c-Cbl ubiquitinate different lysine sites of STAT5.

Fig. 6

The ubiquitination of STAT5a (A) and STAT5b (B) by Cbl-b and c-Cbl in HEK293T cells. C Immunoprecipitation analysis of ubiquitination of STAT5b by Cbl-b in HEK293T cells transfected with Flag-STAT5b (WT or mutant). D Immunoprecipitation analysis of linear ubiquitination of STAT5b by c-Cbl in HEK293T cells transfected with Flag-STAT5b (WT or mutant). E Immunoprecipitation analysis of linear ubiquitination of STAT5a by Cbl-b in HEK293T cells transfected with Flag-STAT5a (WT or mutant). F Immunoprecipitation analysis of linear ubiquitination of STAT5a by c-Cbl in HEK293T cells transfected with Flag-STAT5a (WT or mutant). Data are pooled from three independent experiments.