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. 2022 Mar 30;5:287. doi: 10.1038/s42003-022-03241-y

Fig. 2. Naked mole-rats (NMRs) do not develop 7,12-dimethylbenz[a]anthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (TPA)-induced tumours despite the induction of cellular damage.

Fig. 2

a Schematic diagram for carcinogenesis assessment by DMBA/TPA treatment on the back skin. b Kaplan–Meier curves of tumour-free mice and NMRs after DMBA/TPA treatment. n = 6 animals per species. c Gross appearance and haematoxylin and eosin (HE) staining of mouse papillomas at 20 weeks and NMR skin at 55 weeks after starting DMBA/TPA treatment. Scale bars: 1 cm (upper) and 100 μm (lower). Inset is a higher magnification of NMR skin (scale bar: 50 μm). d Schematic diagram for investigating short-term responses to DMBA treatment on the back skin. e Quantification of phospho-Histone H2A.X (pH2AX)-positive cells at 24 h after DMBA treatment. f Schematic diagram for investigating short-term responses to DMBA/TPA treatment on the back skin. g Immunohistochemical staining and quantification of Ki67-positive cells in NMR skin at 2 weeks after starting DMBA/TPA treatment. Scale bar: 50 μm. For quantification of (e and g), data are presented as the mean ± SD of n = 4 (for mouse) or n = 3 (for NMR) animals. Log-rank test for (b). Unpaired t-test versus untreated control (Ctrl) for (e and g).