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. 2022 Mar 30;5:287. doi: 10.1038/s42003-022-03241-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Venn diagram showing the number of genes upregulated in both mouse and NMR skin upon lipopolysaccharide (LPS) treatment; genes upregulated specifically in mouse or NMR skin upon exposure to 3-methylcholanthrene (3MC, 1 week) and UV; and enriched pathways of 3MC-UV Mouse-DEGs. b Multiple alignments of receptor-interacting kinase 3 (RIPK3) and mixed lineage kinase domain-like (MLKL) sequences from the NMR, Damaraland mole-rat (DMR), guinea pig (GP), rat, human, and mouse. Frame-shift mutations and premature stop codons in the NMR sequence are boxed. Reading frames for the NMR and mouse sequences are indicated. The functional domains are shown above the alignments. c Cell death analysis in fibroblasts treated with a combination of TNF-α (T), cycloheximide (C), z-VAD-fmk (Z), or Nec-1 (N). Data are presented as the mean ± SD of n = 3 independent experiments. d Immunofluorescence staining of high mobility group box-1 protein (HMGB1) in skin at 1 week after 3MC-injection. Scale bar: 10 μm. e Quantification of cytoplasmic HMGB1 in skin after each treatment. Data are presented as the mean ± SD of n = 3 animals for each species. One-way ANOVA with Tukey’s multiple comparison test for (c) and Dunnett’s multiple comparisons test versus untreated control (Ctrl) for (e).