Fig. 4. UV-induced genotoxic stress increases global antitermination.

a Scatterplot comparing the NTS RPKM (RNA-seq) in WT vs WT + UV (120 J/m²) cells. Genes are colored based on their fold increase after UV exposure compared to without UV. Blue genes had a greater than 4-fold increase in antisense transcription and yellow genes had a 2-4-fold increase in antisense transcription. b The distribution of the log₂ termination efficiency (see methods) for each Rho-dependent terminator in WT and WT + UV cells (top). Meta-analysis of the average readthrough past Rho-dependent termination sites in WT and WT + UV cells. The dashed line represents the termination site (bottom). c Schematic showing the location of the qPCR primers A1 and A2 relative to the RUT and termination site (top). The A1/A2 fold-change at three different native Rho terminators after UV exposure (bottom). d The A1/A2 fold change at the proA terminator after exposure to a high or low UV dose. e The A1/A2 fold change at the proA Rho-depended terminator after high or low UvrAB induction. Values are means ± SD from three independent replicates.