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. 2022 Mar 17;13:789008. doi: 10.3389/fendo.2022.789008

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Copyright © 2022 Song, Yu, Zhang, Li, Chen, Cai and Yu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The mRNA expression of mitochondrial functional genes in rat ovarian GCs was detected. A total of 10 mitochondrial functional related genes were analyzed by Real-time PCR. The results showed that the mRNA expressions of gene NDUFB8 (0.40 ± 0.08) and ATP5j (0.40 ± 0.17) in PCOS model rats were significantly decreased compared with those in control group ones (NDUFB8, 1.00 ± 0.22; ATP5j, 1.00 ± 0.18). However, other genes including SDHB, ATP5h, ATP5a1, Opa1, UQCRC2, COXII, NDUFV2 and Mfn1 were not obviously different between two groups. (B) Frequency of mtDNA 4834-bp deleted mitochondria detected. According to Real-time PCR analysis, there was no one with mtDNA 4834-bp deletion in both control and PCOS model group rats. The frequency of mtDNA 4834-bp deletion in both two groups was 0.00%. According to the amplification curve and melting curve, the int primer pair had specific amplification in the PCOS model group, while the del primer pair had no specific amplification, verified the results from both positive and negative perspectives. (C) The protein expressions of mitochondrial functional genes NDUFB8 and ATP5j were detected. According to Western Blot analysis, the protein expression of mitochondrial functional genes NDUFB8 and ATP5j in the ovarian GCs of the PCOS model group rats were obviously decreased compared with those in the control group. The results were consistent with those of Real-time PCR analysis. *P < 0.05.