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. 2022 Mar 17;13:722053. doi: 10.3389/fimmu.2022.722053

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Copyright © 2022 Xia, Xu, Ai, Zhu, Geng, Niu, Zhu, Zhou, Huang and Shi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Macrophage recruitment in in-vitro infection experiments. (A) A systematic study of macrophage recruitment using 24-well transwell chambers (8.0-μM membrane). (B) GFP⁺ ANA-1 recruitment in transwell assays. GFP⁺ macrophages were inoculated into the upper chambers, while A549 cells or ANA-1 macrophages were inoculated into the lower chambers and infected with influenza PR/8/34. Scale bar: 30 μm. (C) The GFP⁺ ANA-1 macrophages in the lower chambers were enumerated to evaluate the extent of trans-membrane recruitment of macrophages. No fewer than three fluorescent images were captured and analyzed per group. **p < 0.01.