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Western blot of strains from Fig 6D. Expression of each construct was observed for each fusion protein by anti-HA Western blotting in cells cultured in EMM5S (no thiamine) and is reduced to undetectable levels after 22 h of culture in EMM5S with 15 μM thiamine. A Coomassie stained gel is shown as a loading control. The position of molecular weight markers (kD) is shown.
