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. 2022 Mar 17;13:816952. doi: 10.3389/fimmu.2022.816952

Figure 6.

Figure 6

Effect of PBM on the Notch1-HIF-1α/NF-κB signalling pathway in M1 macrophages. (A) The mRNA expression levels of Notch signalling pathway-related genes were detected in BMDMs by RT–PCR (n = 3 per group). (B) The mRNA expression levels of Notch1 and HIF-1α were detected by RT–PCR in macrophages in the control (Ctrl), L+I (LPS + IFN-γ), and PBM (LPS + IFN-γ + PBM) groups (n = 3 per group). (C, D) Protein levels of the cleaved transmembrane/intracellular region of Notch1 (NTM) in the indicated groups were determined by western blotting 24 and 48 hours after the first PBM intervention (n = 3 per group at each time point). (E) Protein levels of HIF-1α, NF-κB and p-NF-κB were detected by western blotting of macrophages in the control (Ctrl), L+I (LPS + IFN-γ) and PBM (LPS + IFN-γ + PBM) groups (n = 3 per group). (F) Immunofluorescence of Notch-1 (red) and CD86 (M1 macrophage marker; green) in macrophages from different treatment groups (n = 3 per group). *P < 0.05, **P < 0.01, ***P < 0.001.