Fig. 2.

Exogenous CEACAM5/6 interacts with membrane-bound CEACAM6 in lung cancer cells. (A) Immunoblotting for CEACAM6 using the lysate of various lung cancer cells maintained in 5% fetal bovine serum (FBS) supplemented media. (B) Immunoblotting of culture soup of the indicated lung cancer cells incubated in growth media supplemented with 5% FBS. (C) Kernel density plot of plasma CEACAM6 concentrations from 29 lung adenocarcinomas and 11 control patients. (D) Interaction and co-localization of endogenous CEACAM6 and exogenous His-Tagged CEACAM5/6 in lung cancer cells Co-localization results are shown in yellow. (E) Histogram showing the quantification of colocalization using Mander's colocalization index. *p < 0.05, **p < 0.01, ***p < 0.001 (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.).