Fig. 3. Reversal of Tm through a competition of counterion release and preferential hydration. The data marked by red points have been measured by Senske et al. for the unfolding of RNase A at pH = 5 in presence of an increasing concentration of NaCl.16 The solid line marks the fit by theory with the parameters Δnci = −0.60 and Δw = −31.7 (eqn (23), the term quadratic in ΔT has been neglected). The green dashed line marks the temperature T0m. See text for further explanation.
