FIG. 6.

SDS-PAGE analysis of C. albicans incubated with histatin 5. Cells were incubated for 10 min (lanes 1), 30 min (lanes 2), or 60 min (lanes 3) with histatin 5 (65 nmol · ml⁻¹) and centrifuged. The resulting supernatants (A, C, E, G, and I) and pellets (B, D, F, H, and J) were analyzed by SDS-PAGE on 16% gels. Lanes 4, 60-min control without histatin 5; lanes 5, histatin 5 standard (10 μg). (A and B) Untreated wild-type cells; (C and D) wild-type cells treated with 25 mM sodium azide; (E and F) wild-type cells treated with 100 μM CCCP; (G and H) petite 1; (I and J) petite 2. Gels were stained with Coomassie brilliant blue R250.