Fig. 4.

FUT1 knockdown reversed the inhibitory effects of XIST overexpression on chondrogenic differentiation of BMSCs. (A–B) The mRNA (A) and protein level (B) of FUT1 in BMSCs was determined by qRT-PCR and western blot after 0, 7 and 14 days of chondrogenic culture. BMSCs were transfected with pc-XIST or si-FUT1 alone or co-transfected with pc-XIST and si-FUT1 for 24 h, and undergo chondrogenic induction for 14 days. The acidic mucopolysaccharide (C), mineralized nodule formation (D) and protein levels of Sox9, ACAN and COL2A1 (E) in BMSCs were measured by Alcian blue staining, Alizarin red S staining or western blot, respectively.