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Silence of XIST alleviated the symptoms of cartilage injury in OA mice. Mice were randomly divided into four groups: Sham, OA, OA+si-NC and OA+si-XIST groups. (A) Representative images of cartilage sections after Safranin O-fast and HE staining. (B) Sox9, ACAN and COL2A1 expression in cartilage were assessed by IHC. (C) The protein levels of Sox9, ACAN and COL2A1 in cartilage were measured by western blot. (D) qRT-PCR analysis of XIST, TAF15 and FUT1 RNA levels in cartilage.
