Skip to main content
. 2021 Oct 27;188(4):1900–1916. doi: 10.1093/plphys/kiab492

Figure 1.

Figure 1

Expression profiling of OsWRKY5. A, Relative OsWRKY5 mRNA levels in response to various abiotic stresses. WT seedlings were grown on half-strength MS phytoagar media for 10 d in a growth chamber under LD conditions (14-h light at 30°C/10-h dark at 25°C) and then incubated in half-strength MS liquid medium supplemented with 1-mM ACC, 100-µM ABA, 100-mM NaCl, or 100-mM mannitol for 6 h, or dehydrated by 6-h air drying. Seedlings incubated in half-strength MS liquid medium without treatment were used as mock control. WT leaves were harvested at 0 and 6 h of treatment (HT). B, OsWRKY5 is differentially expressed in various organs of WT plants (japonica cultivar ‘Dongjin’), including flag leaf (FL), leaf blade (LB), leaf sheath (LS), lamina joint (LJ), stem (S), node (N), internode (IN), root (R), tiller base (TB), and panicle (P). Seedling stage: WT seedlings were grown in a growth chamber for 10 DAG under LD conditions (14-h light at 30°C/10-h dark at 25°C). Heading stage: WT plants were grown in a paddy field for 120 DAS under NLD conditions (≥14-h light/day). Relative OsWRKY5 transcript levels were determined by RT-qPCR and normalized to OsUBIQUITIN5 (OsUbq5; Os01g22490). Relative expression was calculated using the ΔΔCT method. Different letters (A) indicate significantly different values according to a one-way ANOVA and Duncan’s least significant range test (P < 0.05). Mean and standard deviation values were obtained from three biological replicates. These experiments were repeated three times with similar results.