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. 2021 Oct 27;188(4):1900–1916. doi: 10.1093/plphys/kiab492

Figure 4.

Figure 4

The oswrky5-2 mutant shows increased ABA sensitivity. A and B, WT, oswrky5-2, and oswrky5-D seeds were germinated on half-strength MS phytoagar medium for 3 d and then grown on half-strength MS phytoagar medium containing 0-, 2-, or 5-µM ABA for 10 d under LD conditions. Seedlings grown on half-strength MS plates with 0-µM ABA were used as mock control. White bars = 3 cm. A, Growth phenotypes and (B) the shoot length of the WT, oswrky5-2, and oswrky5-D plants were measured after 10-d ABA treatment (n = 10). Asterisks above oswrky5-2 and oswrky5-D data points indicate a statistically significant difference from the WT, as determined by Student’s t test (*P < 0.05, **P < 0.01, ***P < 0.001). C, Effect of ABA on stomatal aperture. Detached leaves of 3-week-old WT, oswrky5-2, and oswrky5-D plants grown in the greenhouse under NLD conditions were incubated in 3-mM MES buffer (pH 5.8) containing 100-µM ABA for 1 h. Detached leaves floated on 3-mM MES buffer (pH 5.8) without phytohormones were used as mock control (79, 81, and 77 stomata were evaluated in the WT, oswrky5-2, and oswrky5-D under mock control; 72, 81, and 77 stomata were evaluated in the WT, oswrky5-2, and oswrky5-D under ABA treatment, respectively). Different letters indicate significantly different values according to a one-way ANOVA and Duncan’s least significant range test (P < 0.05). Mean and standard deviation values were obtained from three biological replicates. These experiments were repeated three times with similar results.