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. 2021 Dec 27;188(4):2325–2341. doi: 10.1093/plphys/kiab604

Figure 5.

Figure 5

OsPIP2;2 associates with OsmaMYB. A, Associations between OsPIP2;2 and OsmaMYB in the split-ubiquitin yeast two-hybrid system. Yeast cells co-transformed with bait and prey vectors were grown on medium supplemented with 10 mM of 3-amino-1,2,4-triazole. B, Co-IP of OsPIP2;2 and OsmaMYB after co-expression in N. benthamiana. The indicated constructs were co-expressed in N. benthamiana by A. tumefaciens. Total proteins were extracted 2 d postinfiltration, and subjected to anti-FLAG immunoprecipitations. C and D, Association between OsPIP2;2 and OsmaMYB indicated by the split-LUC complementation assay. The indicated constructs were co-expressed in N. benthamiana leaves and then LUC activities were examined in both qualitative (C) and quantitative assays (D). Cluc-CPR5 and BIK1-Nluc were used as the negative control. Cluc-XLG2 and BIK1-Nluc served as the positive control. Data are shown as means ± SEM (n = 8). Lowercase letters indicate significant differences at P ≤ 0.01, Duncan’s multiple range tests and one-way ANOVA.