TABLE 3.
Effects of acidic terpenoids on macromolecular synthesis in C. albicans MY1055a
| Compound | MIC (μg/ml) | Concn (μg/ml) required to inhibit synthesis by 50%
|
||||
|---|---|---|---|---|---|---|
| Glucan | Chitin | Mannan | RNA | Protein | ||
| L-733560 | 0.1 | 0.04 | >0.5 | >0.5 | 20.0 | >20.0 |
| Nikkomycin | 2.0 | >50.0 | 0.2 | >50.0 | >50.0 | >50.0 |
| Tunicamycin | 2.5 | >20.0 | >20.0 | 1.0 | >20.0 | >20.0 |
| Digitonin | 2.5 | 0.6 | 0.6 | 0.6 | 5.0 | 10.0 |
| Enfumafungin | 0.2 | 0.05 | >25.0 | >25.0 | 10.0 | >20.0 |
| Ascosteroside | 0.2 | 0.05 | >50.0 | >50.0 | >40.0 | >40.0 |
| Ergokonin A | 0.7 | 0.2 | >25.0 | >25.0 | 10.0 | >100.0 |
| Arundifungin | 5.0 | 6.25 | >50.0 | >50.0 | 12.5 | >100.0 |
a
The MICs were determined in CM at the same cell density used in the macromolecular labeling experiments. The MICs of ascosteroside and nikkomycin Z are the lowest concentrations required to reduce the growth of Candida by 50% compared to the growth of untreated cells. The effect on growth relative to that on the growth of untreated control cells was recorded visually after 4 h at 30°C. The effect on macromolecular synthesis was determined as described in Materials and Methods.