TABLE 4.
Effect of assay conditions on potencies of L-733560 and acidic terpenoids in glucan synthase assay of C. albicans MY1055
| Compound | Glucan synthase IC50 (μg/ml)
|
Concn (μg/ml) required to inhibit whole-cell glucan synthesis to 50% of that for the control | |
|---|---|---|---|
| Standard assay | Modified assay | ||
| L-733560 | 0.001 | 0.002 | 0.04 |
| Enfumafungin | 1–>25 | 1.0 | 0.05 |
| Ascosteroside | >25.0 | 7.0 | 0.05 |
| Ergokonin A | 3.0 | 0.2 | |
| Arundifungin | 10.0 | 6.0 | 6.25 |
a
Glucan synthase assays were performed as outlined in Materials and Methods.