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. Author manuscript; available in PMC: 2022 Mar 31.
Published in final edited form as: Cell Signal. 2020 Feb 26;70:109588. doi: 10.1016/j.cellsig.2020.109588

Fig. 5. Chronic WNT stimulation induces and cellular senescence in epithelial cells.

Fig. 5.

(A-C)Preciscion cut lung slices (PCLS) were prepared from young mice and treated with (A) 2 μM CHIR for 7 days. n = 5, or (B) conditioned medium from WNT3A-overexpressing L-cells (WNT3A CM; 1:1) for 7 days, n = 3. (A-B) qPCR analysis for WNT target gene Axin2 and senescence markers Cdkn2a and Cdkn1a was performed and normalized to Hprt levels. (C) Representative images of immunofluorescence staining for P21 and CDH1 (E-CAD) in PCLS prepared from young mice and treated with WNT3A CM for 7 days. Fluorescent images represent a 400 × magnification. The scale bar represents 50 μm. Representative of n = 3. Significance was assessed by paired Student's t-test (A-B). Significance: *p < .05, **P < .01.