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Primer sequences of genes for quantitative PCR.
| Gene | Primer sequence (5′-3′) |
|---|---|
| SLC7A11 | |
| Forward | GGTCCATTACCAGCTTTTGTACG |
| Reverse | AATGTAGCGTCCAAATGCCAG |
| GPX4 | |
| Forward | GAGGCAAGACCGAAGTAAACTAC |
| Reverse | CCGAACTGGTTACACGGGAA |
| ACSL4 | |
| Forward | CATCCCTGGAGCAGATACTCT |
| Reverse | TCACTTAGGATTTCCCTGGTCC |
| β-actin | |
| Forward | GACCTGTACGCCAACACAGT |
| Reverse | CTCAGGAGGAGCAATGATCT |
SLC7A11, solute carrier family 7 member 11; ACSL4, acyl-CoA synthase long chain family member 4; GPX4, glutathione peroxidase 4.
