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. 2022 Mar 17;13:841099. doi: 10.3389/fimmu.2022.841099

Figure 4.

Figure 4

FCGR1A SNV rs1050204 (c.970G>A or p.D324N) alleles affect CD64-mediated phagocytosis and degranulation. (A) Effect of the SNV rs1050204 (FcγRIA-p.D324N) on FcγRIA-mediated phagocytosis. P388D1 cell lines expressed equivalent levels of FcγRIA-p.324D (rs1050204G) and FcγRIA-p.324N (rs1050204A) (left panel). FcγRIA-p.324N mediated significantly higher levels of phagocytosis (*Student’s t-test P = 0.0056, n = 4). (B) Effect of the SNV rs1050204 (p.D324N) on FcγRIA-mediated degranulation. Stably transfected and RBL cell lines express FcγRIA-p.324D (rs1050204G) and FcγRIA-p.324N (rs1050204A) alleles at comparable levels. FcγRIA-mediated degranulation induced by anti-CD64 mAb 32,2 F(ab’)2 crosslinking was significantly higher in the RBL cells with FcγRIA-p.324N allele than those with FcγRIA-p.324D (*Student’s t-test, P < 0.01, n = 4). No degranulation was induced by the irrelevant mIgG F(ab’)2 in the stable RBL cells expressing either p.324D and p.324N alleles.