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. 2022 Mar 17;13:831397. doi: 10.3389/fgene.2022.831397

FIGURE 5.

FIGURE 5

IGF2BP1 is upregulated in LUAD cells, and the inhibition of IGF2BP1 can mitigate the promotion of overexpressed HOXA11-AS on LUAD cell proliferation. (A) Expression of target gene IGF2BP1 in normal and tumor group was depicted in boxplot (blue refers to normal tissue, orange refers to tumor tissue). (B) Expression of IGF2BP1 in tumor tissue (n = 30) and adjacent normal tissue (n = 30). (C) Expression of IGF2BP1 in various stages of LUAD (stage i: n = 279; stage ii: n = 124; stage iii: n = 85; stage iv: n = 26). (D) Relationship between IGF2BP1 and survival rate was estimated with red for high IGF2BP1 expression and blue for low IGF2BP1 expression. (E) qRT-PCR was conducted for the assessment of IGF2BP1 mRNA in normal cell line HBE4-E6/E7 and LUAD cell line A549. (F) IGF2BP1 mRNA in transfected cells was evaluated in qRT-PCR assay. (G) By carrying out CCK-8 assay, cell viability in each group was estimated. (H) Through Western blot, the expression of proliferation of Ki67 as well as IGF2BP1 in each group of cells was tested; *p < 0.05.