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. 2022 Mar 30;12(3):49. doi: 10.1038/s41408-022-00650-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Exemplary flow cytometric image of one CLL case positive for expressing IGLV3-21^R110 (CLL374). The images were created using FlowJo™ Software (BD Biosciences). The CD19 + /CD5 + cell fraction of CLL374 was sorted and subjected to heavy and light chain NGS for confirmation of CLL clone sequences. B Sites of somatic hypermutation in IGHV and IGLV genes of clone 1 and 2 of CLL374 with discordant IGLV-J rearrangements. Clone 1 and 2 were found in bulk sequencing of genomic DNA isolated from PBMCs as well as in the CD5 + /CD19 + cell fraction sorted via FACS (A). C Schematic representation of clonal trajectories leading to different CLL subclones in patient CLL374.