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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: J Comp Neurol. 2021 Dec 15;530(8):1213–1230. doi: 10.1002/cne.25270

Figure 8. NFI’s in the pig retina.

Figure 8.

scRNA-seq was used to identify patterns of expression of NFIs in adult pig retina. Four different scRNA-seq libraries were aggregated for a total of 15,236 cells (a). UMAP clustered cells were identified based on cell-distinguishing markers (b). The expression of NFIA, NFIB, NFIC and NFIX is illustrated in UMAP heatmap plots (c). Vertical sections of central and peripheral regions of the retina were labeled with antibodies to NFIA (green; d-f), NFIB (green; g), Sox2 (red; d and g), Otx2 (red; e), or AP2α (red; f). Arrows indicate the nuclei of MG, small double arrows indicate the nuclei of an unidentified cell type in the NFL, hollow arrow heads indicate nuclei of amacrine cells, and small double-arrow heads indicate nuclei of bipolar cells. The calibration bar represents 50 μm. Abbreviations: ONL – outer nuclear layer, INL – inner nuclear layer, IPL – inner plexiform layer, GCL – ganglion cell layer.