Table 1.
List of antibodies, working dilution, clone/catalog number and source.
| Antibody | Dilution | Host | Clone/Catalog number | Source |
|---|---|---|---|---|
| NFIA | 1:300 | Rabbit | HPA006111 | Atlas Antibodies |
| NFIB | 1:300 | Rabbit | LS-B13531 | LSBio |
| Sox2 | 1:1000 | Goat | KOY0418121 | R&D |
| Sox9 | 1:2000 | Rabbit | AB5535 | Millipore |
| CD45 | 1:200 | Mouse | HIS-C7 | Prionics |
| Nkx2.2 | 1:100 | Mouse | 74.5A5 | DSHB |
| Neurofilament | 1:100 | Mouse | RT97 | DSHB |
| AP2-alpha | 1:50 | Mouse | AP2A | DSHB |
| Brn3a | 1:50 | Mouse | MAB1585 | Chemicon |
| Islet1 | 1:50 | Mouse | 40.2D6 | DSHB |
| Otx2 | 1:1000 | Goat | AF1979 | RD Systems |
| PCNA | 1:1000 | Mouse | M0879 | Dako |
| p21-CIP1 (CDKN1A) | 1:200 | Rabbit | B9242 | LSBio |