Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 30;9:120. doi: 10.1038/s41597-022-01242-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Validation of the MEA recordings of neuronal networks and signal analysis. (a) The quality of hPSC-derived neural cultures was verified with immunocytochemical staining of pre- and postsynaptic markers (synaptophysin in red and PSD-95 in green) overlaid with neuron-specific b-tubulin staining (white). DAPI stains cell nuclei. A phase contrast image of hPSC-derived neurons on the MEA is used to illustrate the formation of networks over the electrode area. (b) The performance of spike detection in recognizing the typical low-amplitude signal of hPSC-derived networks was confirmed. The detected spikes are illustrated with red dots (top) and red bars (bottom). A single channel burst detection algorithm successfully detected quasiperiodic burst activity, which is commonly observed in mature neuronal cultures. The detected bursts are labeled with black lines on the red spike bars (bottom). (c) The MEA signal was associated with action potentials, as it was blocked by the voltage-gated sodium channel antagonist tetrodotoxin (TTX). The number of detected spikes (red bars) decreased considerably (bottom) compared to the baseline recording (top).