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. 2022 Mar 30;8:144. doi: 10.1038/s41420-022-00954-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Expression of five most upregulated and downregulated circRNAs in five paired GC tumors and normal tissues. B Expression of hsa_circ_0007967 in 47 pairs of GC tissues and normal tissues. C Expression of hsa_circ_0007967 in GC cell lines and normal gastric epithelial cell line. D The location of hsa_circ_0007967 in chromatin and its structure. E Sanger sequencing confirming the back splicing junction site. F The RNase R digestion assay suggesting hsa_circ_0007967 is resistant to RNase R. G The use of act-D (1 μg/μl) demonstrating that hsa_circ_0007967 is stable than its linear form. H Northern blotting of hsa_circ_0007967 and its linear form in cDNA and gDNA amplified by convergent and divergent primers. I Location of hsa_circ_0007967 in BGC823 cells detected by FISH. J QPCR analysis of nuclear and cytoplasmic fractions conforming that hsa_circ_0007967 is located in cytoplasm.