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LC-MS chromatograms of ICT compounds incubated with CYP1A1 (1A1) or CYP2W1 (2W1) for 30 minutes; M6 = spirocyclized product. (A) ICT2700 R/S CYP1A1 metabolism. (B) ICT2700 R/S CYP2W1 metabolism. (C) ICT2726 R/S CYP1A1 metabolism (D) ICT2726 R/S CYP2W1 metabolism. AU, absorbance units; ESI+, electrospray ionization in positive mode; MS, mass; M+H, singly protonated mass; RT, retention time.
