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. 2022 Mar 29;9(3):e1162. doi: 10.1212/NXI.0000000000001162

Figure 2. The Novel CBA With α3β4-nAChR–Transfected HEK293 Cells Detects Antibodies in Sera With High or Low RIPA Antibody Levels Specifically in Patients With AAG.

Figure 2

HEK293 cells expressing α3β4, α3β2-nAChRs, or control AQP4 molecule were incubated with sera from patients with AAG (of high or low RIPA antibody levels) or from a patient with POTS and stained with anti-human IgG (red). It is shown that both patients with AAG showed positive staining, independently of the RIPA antibody level, whereas the patient with POTS gave no staining. The AAG RIPA-high, AAG RIPA-low, and POTS RIPA-low patients are patients no. 6 (RIPA 1.4 nM, CBA 1/1,000), 18 (RIPA 0.05 nM, CBA 1/10), and 16 (RIPA 0.13 nM, CBA negative) of Table 1, respectively. Scale bar: 20 μm. AAG = autoimmune autonomic ganglionopathy; CBA = cell-based-assay; POTS = postural orthostatic tachycardia syndrome; RIPA = radioimmunoprecipitation assay.