Figure 4. Prophylactic Siponimod Therapy Reduces Immune Cell Infiltration and Prevents Demyelination.

(A) Longitudinal sections of optic nerves of C57Bl/6J mice were stained for Iba1, CD3, and MBP 90 days after myelin oligodendrocyte glycoprotein fragment 35-55 immunization; dotted lines indicate areas of demyelination, scale bar = 50 µm. Quantitative analyses of microglial activation (Iba1, B) by fluorescence intensity measurement, T-cell infiltration (CD3 score, C), and myelin status (MBP score, D). One optic nerve per mouse was included. All graphs represent the pooled mean ± SEM, and gray dots show individual data points (n = 6 animals per group out of 2 independent experiments), with *p < 0.05, **p < 0.01, and ***p < 0.001 by ANOVA with the Dunnett post hoc test compared with myelin oligodendrocyte glycoprotein untreated mice. §Few data points out of axis limits. ANOVA = analysis of variance.