. 2022 Mar 30;23:124. doi: 10.1186/s12882-022-02753-3

Table 1.

Primer sequences for RT-qPCR

Primers	Sequence (5'-3')
hsa-NSD2	Forward: TGTGTGAGCTGCCATGCTTCCA
hsa-NSD2	Reverse: TGAGCATCCTGCTGCCAGACAA
hsa- METTL3	Forward: CTATCTCCTGGCACTCGCAAGA
hsa- METTL3	Reverse: GCTTGAACCGTGCAACCACATC
mmu-NSD2	Forward: TTCCGCTGTCCTCTCCATAGCT
mmu-NSD2	Reverse: GCAATCACCGAACATCCTGCTG
mmu-METTL3	Forward: CAGTGCTACAGGATGACGGCTT
mmu-METTL3	Reverse: CCGTCCTAATGATGCGCTGCAG
mmu-YTHDF1	Forward: GCATCAGAAGGATGCAGTTCATG
mmu-YTHDF1	Reverse: GATGGTGGATAGTAACTGGACAG
hsa-GAPDH	Forward: GTCTCCTCTGACTTCAACAGCG
hsa-GAPDH	Reverse: ACCACCCTGTTGCTGTAGCCAA
mmu-GAPDH	Forward: CATCACTGCCACCCAGAAGACTG
mmu-GAPDH	Reverse: ATGCCAGTGAGCTTCCCGTTCAG

Note: RT-qPCR Reverse transcription-quantitative polymerase chain reaction, hsa homo sapiens, mmu mus musculus, NSD2 Nuclear receptor-binding SET domain protein 2, METTL3 Methyltransferase like 3, YTHDF1 YT521-B homology domain family member 1, GAPDH glyceraldehyde-3-phosphate dehydrogenase