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. 2022 Mar 17;13:810230. doi: 10.3389/fmicb.2022.810230

FIGURE 1.

FIGURE 1

CM improves duodenal morphology and physical barrier function. (A) HE-stained section of duodenum, (black) represents the height of villi, (yellow) represents the depth of crypts. (B) The villus height (n = 5). (C) The crypt depth (n = 5). (D) The ratio of the villus height and crypt dept (n = 5). (E) RT-qPCR analysis of tight junction proteins ZO-1, occludin, and cladin-1 (n = 4). (F,G) Western blot analysis of tight junction proteins ZO-1, occludin, and cladin-1 (n = 3). Control, pigs fed a basal diet; CM, pigs fed the basal diet supplemented with CM at 300 mg/kg. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01.