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CM inhibits the TLR4/MyD88/NF-κB signaling pathway. (A) RT-qPCR analysis of the mRNA expression levels of TLR4, MyD88, TRAF6, and NF-κB (n = 4). (B) Western blot analysis of the protein expression levels of TLR4, MyD88, and TRAF6 (n = 3). (C) Western blot analysis of the protein expression levels of p-NF-κB p65 and p-IκB-α (n = 3). Control, pigs fed a basal diet; CM, pigs fed the basal diet supplemented with CM at 300 mg/kg. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01.
