TABLE 3.
Primers sequences for RT-qPCR.
| Genes | Primer sequence (5′–3′) | Product length (bp) | GenBank accession |
| ZO-1 | F: CAGCCCCCGTACATGGAGA R: GCGCAGACGGTGTTCATAGTT | 114 | XM_021098896.1 |
| Occludin | F: CTACTCGTCCAACGGGAAAG R: ACGCCTCCAAGTTACCACTG | 158 | NM_001163647.2 |
| claudin-1 | F: GCCACAGCAAGGTATGGTAAC R: AGTAGGGCACCTCCCAGAAG | 140 | NM_001244539.1 |
| MUC-2 | F: CTGCTCCGGGTCCTGTGGGA R: CCCGCTGGCTGGTGCGATAC | 101 | XM_021082584.1 |
| TLR4 | F: ATATGGCAGAGGTGAAAGCAC R: GAAGGCAGAGATGAAAAGGGG | 125 | NM_001113039.2 |
| MyD88 | F: TGGTGGTGGTTGTCTCTGATGA R: TGGAGAGAGGCTGAGTGCAA | 80 | NM_002468 |
| TRAF6 | F: GGGAACGATACGCCTTACAA R: CTCTGTCTTAGGGCGTCCAG | 174 | NM_001105286.1 |
| NF-κB | F: CTCGCACAAGGAGACATGAA R: ACTCAGCCGGAAGGCATTAT | 147 | NM_001048232.1 |
| β-actin | F: GGACTTCGAGCAGGAGATGG R: AGGAAGGAGGGCTGGAAGAG | 138 | XM_021086047.1 |
F, forward; R, reverse; ZO-1, zona occluden-1; MUC-2, mucin-2; TLR4, Toll-like receptor 4; MyD88, myeloid differentiation factor 88; TRAF6, tumor necrosis factor receptor-associated factor 6; NF-κB, nuclear factor-kappa B.