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. 2022 Mar 17;13:799942. doi: 10.3389/fmicb.2022.799942

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Copyright © 2022 Zhang, Yan, Liu, Li, Feng, Xu, Wang, Gao, Yang, Zhao and Xia.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of antibody levels in immunized mice sera. Sera collection from mice immunized with different amounts of RVFV-BLPs. RVFV Gn head-specific IgG (p > 0.05), IgG1, IgG2a, and IgG3 levels in the serum were assessed by indirect ELISA; data are shown as the means ± SD. RVFV Gn head-specific IgG levels in mice immunized with different amounts of RVFV-BLPs (A), and the levels of IgG subtypes in RVFV-BLPs (20 μg) (B), RVFV-BLPs (50 μg) (C) and RVFV-BLPs (100 μg) (D). Dotted line is cutoff values, calculated as OD450 of control × 2.1. Data are shown as the mean ± SEM and were analyzed by an unpaired t-test.