Figure 3.

S-MGB-364/S-MGB-364-NIV is non-genotoxic to macrophages and inhibits topoisomerase I. (a) Inhibition of topoisomerases and gyrases by S-MGB-364. Camptothecin, mAMSA, and ciprofloxacin are controls for the human topoisomerase I, Mtb topoisomerase I, and Mtb gyrase enzymes, respectively. These values are presented as mean ± SD with n = 2. (b) Example gel illustrating the inhibition of Mtb topoisomerase I relaxation by S-MGB-364. (c) CD11b⁺F4/80⁺MHCII⁺ bone-marrow-derived macrophages were treated with S-MGB-364 (10 μM), S-MGB-364-NIV (10 μM), and the positive control H₂O₂ (10 μM) for 4 and 24 h. Intracellular expression of γ-H₂Ax expression was measured by flow cytometry. (a) Data show mean ± SEM of triplicates. ND, not detected. Two-tailed Student’s t-test, **P < 0.01 compared with non-treated.