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. 2022 Mar 17;13:840792. doi: 10.3389/fmicb.2022.840792

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Copyright © 2022 Zhao, Wang, Li, Han, Han and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The growth and motility of X2-3, MT16, and Com-16. (A) X2-3, MT16, and Com-16 growth curves. The X2-3, MT16, and Com-16 strains were cultured in NB medium, adjusted to OD₆₀₀ = 1.0, and then subcultured in fresh NB for 48 h. The OD₆₀₀ values were tested every 4 h post-subculturing. All experiments were repeated at least three times. (B) Twitching motility of X2-3, MT16, and Com-16. The X2-3, MT16, and Com-16 strains were grown for 24 h in NB medium at 28°C and adjusted to OD₆₀₀ = 1.0. Three microliters of each cell sample was dropped onto 0.6% agar plates for the motility tests. The diameters of each colony were measured after 3 days of incubation, and the resulting values were taken to indicate the bacterial motility. Each experiment was performed at least three times. a, not significant compared to X2-3. b, significant difference compared to X2-3.