Figure 1.
The growth and motility of X2-3, MT16, and Com-16. (A) X2-3, MT16, and Com-16 growth curves. The X2-3, MT16, and Com-16 strains were cultured in NB medium, adjusted to OD600 = 1.0, and then subcultured in fresh NB for 48 h. The OD600 values were tested every 4 h post-subculturing. All experiments were repeated at least three times. (B) Twitching motility of X2-3, MT16, and Com-16. The X2-3, MT16, and Com-16 strains were grown for 24 h in NB medium at 28°C and adjusted to OD600 = 1.0. Three microliters of each cell sample was dropped onto 0.6% agar plates for the motility tests. The diameters of each colony were measured after 3 days of incubation, and the resulting values were taken to indicate the bacterial motility. Each experiment was performed at least three times. a, not significant compared to X2-3. b, significant difference compared to X2-3.