Figure 2.

The ability to produce biofilms of X2-3, MT16, and Com-16. Biofilm formation of X2-3, MT16, and Com-16 on glass bottle surfaces after 3 days of incubation in NB medium supplemented with 1% sucrose or glucose. (A) Biofilm formation of X2-3, MT16, and Com-16 on glass bottle surfaces by crystal violet stain. (B) The results of the biofilm formation assays were quantified by measuring the absorbance of the crystal violet stain at 575 nm. Each experiment was performed at least three times. a, not significant compared to X2-3. b, significant difference compared to X2-3. (C) Pellicle formation by X2-3, MT16, and Com-16. All strains were analyzed after 5 days of incubation at 28°C, showing developed pellicles at the interface between the liquid and air in NB medium supplemented with 1% sucrose or glucose.