Table 1.
Bacterial strains, plasmids, and primers applied in this study.
| Strains or plasmids | Relevant characteristics* | Source/Reference |
|---|---|---|
| Lysobacter capsici | ||
| X2-3 | Wide type strain | This study |
| MT16 | The gidA deletion mutant of strain X2-3; Kmr | This study |
| Com-16 | The complemented strain of gidA deletion mutant | This study |
| Escherichia coli | ||
| DH5α | F′ recA, Ф80 d lacZ, and ΔM15 | TransGen |
| S17-1 | Host strain for molecular cloning | This lab |
| E. coli Trans1-T1 | F−φ80 (lacz) ΔM15ΔlacX74hadR (rk−, mk−) ΔrecA1398endA1tonA | TransGen |
| Plasmids | ||
| pMD19-T Simple | Cloning vector; Ampr | Vazyme |
| pEASY-Blunt Simple | Cloning vector; Kmr; Ampr | TransGen |
| PBBR1-MCS5 | Broad-host-range vector with a Plac Promoter, Gmr | Vazyme |
| pKMS1 | 6,400 bp, pUC18 polylinker, mob, oriV, and sacB; Kmr | Zou et al., 2011 |
| pKMS1-AB | pKMS1 carrying 0.998-kb gene fragment harboring two LC_GidA flanking regions (including the upstream and the downstream of LC_GidA); Kmr | This study |
| pBBR1-gidA | pBBR1-MCS5 carrying 1.890-kb gene fragment harboring the intact LC_GidA gene; Gmr | This study |
| PBBR1-gfp | pBBR1-MCS5 carrying 0.72-kb gene fragment harboring the intact gfp gene; Gmr | This study |
| Primer | Sequence (5′-3′; restriction enzyme sites underlined) | Description |
| gidAup-F | 5′-CGGGATCCCCTGAATGCTCCGCAAACTCT-3′ | 689 bp fragment flanking the left of LC_GidA |
| gidAup-R | 5′-TCGGATCATATTCAGCGCTCGACGT-3′ | |
| gidAdown-F | 5′-ACGTCGAGCGCTGAATATGATCCGA-3′ | 309 bp fragment flanking the right of LC_GidA |
| gidAdown-R | 5′-CCAAGCTTGAAGAACAGGCCCAGGTGGA-3′ | |
| gidAF | 5′-CGGAATTCGCTGAATGAACGATCCCTTCTAT-3′ | 1,890 bp LC_GidA gene |
| gidAR | 5’-CGGGATCCTCACGCCACCCGCGAACGC-3′ | |
| gfpF | 5′-CGGAATTCATGGTGAGCAAGGGCGAG-3’ | 720 bp gfp gene |
| gfpR | 5′-CGGGATCCTTACTTGTACAGCTCGTCCATGC-3′ | |
*
Kmr, kanamycin resistance; Ampr, ampicillin resistance; and Gmr, gentamicin resistance.