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. 2022 Mar 17;13:840792. doi: 10.3389/fmicb.2022.840792

Table 2.

Primers used in RT–qPCR.

Primer Sequence (5′-3′) Description
16s rRNA-F 5′-GCTCGTGTCGTGAGATGTT-3′ RT–qPCR
16s rRNA-R 5′-TGTAGCCCAGGTCATAAGG-3′
RT-pilA-F 5′-CAGCAAGGCTTTACCCTCATC-3′ RT–qPCR
RT-pilA-R 5′-TTCTTGGTGCGGATCGTGTAG-3′
RT-flgD-F 5′-CGACCAGGAAGATTTCATCAAGC-3′ RT–qPCR
RT-flgD-R 5′-GATTGCTCCAGCGAGGTGAAC-3′
RT-fliF-F 5′-CTCAACAACGAGGAGTTCAAGG-3′ RT–qPCR
RT-fliF-R 5′-TGGCTGGAATTGATCCGCTTG-3′
RT-flhB-F 5′-GTTCACCACCCATCCGCTCAA-3′ RT–qPCR
RT-flhB-R 5′-CATCAGGCACTTGACCACCAG-3′
RT-fliQ-F 5′-TTGGTCGTCGGTCTGCTGATT-3′ RT–qPCR
RT-fliQ-R 5′-AGCTTGGGCACGAAGGTCAAA-3′
RT-fliP-F 5′-TGCCGCTGAAGATATTGCTGTT-3′ RT–qPCR
RT-fliP-R 5′-CGTCCAGTAACTGCTCAACAAGG-3′
RT-recN-F 5′-AACTGATCCAGACCCATGCC-3′ RT–qPCR
RT-recN-R 5′-AATGCATCCTTGCCGATCCA-3′
RT-radC-F 5′-CTGTTCCACGGCACCATCAA-3′ RT–qPCR
RT-radC-R 5′-AAATGATCGAGCAGGCGGAT-3′
RT-gyrA-F 5′-CACCGGCAGCGATCTTATCT-3′ RT–qPCR
RT-gyrA-R 5′-GACAGCCAGTCGGAATGGAA-3′
RT-dnaA-F 5′-GTGATGTACCTGCGTTCGGA-3′ RT–qPCR
RT-dnaA-R 5′-TGGCGCTTGAACTGATCCAT-3′
RT-rmuC-F 5′-TGTCGAACGAGAAGTACCGC-3′ RT–qPCR
RT-rmuC-R 5′-TTCGACTTCTTCCTGCGCTT-3′
RT-n6amt-F 5′-CCGGCGACATGGACTATCTG-3′ RT–qPCR
RT-n6amt-R 5′-GCACCAGGCTGGAATTGATG-3′
RT-ftsB-F 5′-CTGGCTCGAGGATGACGGG-3′ RT–qPCR
RT-ftsB-R 5’-GTCAGGACGACGGTCGCATA-3′
RT-ftsQ-F 5’-GTCGTCCGTTCCTGTACCTG-3’ RT–qPCR
RT-ftsQ-R 5′-AGTGACTGCCGTAACTGAGC-3’
RT-ftsI-F 5′-ACCGTATTGCGCTTCGACAA-3′ RT–qPCR
RT-ftsI-R 5′-TCGAAGCTGACTTCGCTCAA-3′
RT-pgaA-F 5′-GACGAACTGGTGATGCTCAAC-3′ RT–qPCR
RT-pgaA-R 5′-ATCGGCAGTTGGATGTTCTCG-3′
RT-pgaB-F 5′-CGGTGCTCGGTTACGAATTGC-3′ RT–qPCR
RT-pgaB-R 5′-GAACGGATTGAGGCGGAAGGT-3′
RT-pgaC-F 5′-CACCGAGGACATCGACATCAG-3′ RT–qPCR
RT-pgaC-R 5′-TTCAGGGTTTCAGGCATCAAGA-3′
RT-surA-F 5′-CGAGGACGTGGTCAAGGAAA-3′ RT–qPCR
RT-surA-R 5′-CAGGAAGCGGTTCCACTCTT-3′