Figure 1.

Dose-dependent effects of Sev on ferroptosis in glioma cells. U87 and U251 cells were treated with 1.7%, 3.4%, and 5.1% Sev, respectively. Cell viability was detected using CCK-8 assay in Sev-treated U87 (A) and U251 cells (B). Fe²⁺ concentrations were determined by colorimetric assay in Sev-treated U87 and U251 cells (C). ROS assay combined with flow cytometry was used to observe the content of ROS generation in SEV-treated U87 (D) and U251 cells (E); the ratio of ROS generation was calculated (F). The expression of ferroptosis-associated protein GPX4, ferritin, and transferrin in U87 and U251 cells was detected using Western blotting; GAPDH was used as the internal control (G, H, I). Data were expressed as mean ± standard deviation. *p < 0.05, **p < 0.01, ***p < 0.001, compared with control.