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. 2022 Mar 17;12:859621. doi: 10.3389/fonc.2022.859621

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Copyright © 2022 Xu, Zhang, Chen, Xu, Luo, Yan, Lu, Liu, Ou, Tan, Liang, Chen, Song and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Induction of ferroptosis reversed the effects of ATF4 suppression on Sev-induced ferroptosis in glioma cells. Ferroptosis inducer Erastin was used to incubate in Sev-treated and ATF4-suppressed U87 and U251 cells. Cell viability was detected using CCK-8 assay in U87 (A) and U251 cells (B). Fe2+ concentrations were determined by colorimetric assay in U87 and U251 cells (C). ROS assay combined with flow cytometry was used to observe the content of ROS generation in U87 and U251 cells (D); the ratio of ROS generation was calculated (E). The expression of ATF4 and CHAC1 in U87 and U251 cells was detected using Western blotting; GAPDH was used as the internal control (F, G). Data were expressed as mean ± standard deviation. *p < 0.05, **p < 0.01, compared with control.