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. 2022 Mar 17;13:846770. doi: 10.3389/fmicb.2022.846770

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Copyright © 2022 Wu, Yang, Ru, Qin, Li, Zhang, Zhang, Li, Zhang and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sensitivity of the real-time recombinase-aid amplification (RAA) assay. (A) Amplification curve of the real-time RAA assay over time using a dilution range of 10⁶–10¹ copies/reaction of pASFV/RAA. (B) Reproducibility of the real-time RAA assay. The threshold time is represented as the mean ± standard deviation (SD). The standard regression line was generated based on three datasets. NC, negative control.