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. 2022 Mar 17;10:855097. doi: 10.3389/fcell.2022.855097

FIGURE 2.

FIGURE 2

Elevation of MLC phosphorylation following Cav1 knockdown in TM cells on soft versus hard surface. ((A), Top) MLC phosphorylation was not significantly different between Cav1-deficient and Cav1-competent TM cells when plated on a hard surface (p = 0.224, paired t-test, n = 12 experiments using seven different human TM cell strains). ((A), Bottom) Shown is a representative blot from a single experiment displaying phosphorylated MLC abundance, compared to total MLC protein. ((B), Top) In contrast, MLC phosphorylation was significantly elevated by approximately 37% in Cav1-deficient TM cells compared to Cav1-competent TM cells when plated on a soft surface (*p < 0.0001, paired t-test, n = 24 experiments using 11 different human TM cell strains). ((B), Bottom) Representative blots from a single experiment displaying phosphorylated MLC abundance, compared to total MLC protein. Summary data are displayed as box and whisker plots, which show the minimum, 25th percentile, median, 75th percentile, and maximum values of the dataset, plus all individual data points (black circles). Data are expressed as candidate protein expression (corrected for loading as indicated by α-tubulin abundance) in cells treated with shCAV1, normalized to shSCR treated cells.