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. 2022 Mar 17;13:821976. doi: 10.3389/fmicb.2022.821976

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Copyright © 2022 Yeo, Chong, Chen, Lim, Ng, Yan, Chu, Chow and Alonso.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Infection profiles of EV-A71 WT and mutant strains. RD (A), SH-SY5Y (B), NSC-34 (C) and SK-N-SH (D) cells were infected with the WT S41 strain and the various mutants at MOI of 0.001 (RD and SH-SY5Y), 0.1 (SK-N-SH), and 1 (NSC-34). Supernatants were harvested at the indicated time-points p.i., and the virus titers were quantified by plaque assay. One set representative of two independent experiments is shown. Data were expressed as mean ± SD.