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. Author manuscript; available in PMC: 2022 Mar 31.
Published in final edited form as: Stem Cell Res. 2021 Aug 8;55:102493. doi: 10.1016/j.scr.2021.102493

Figure 2. KLF4 and TFCP2L1 are required for naive pluripotency in 5iLAF.

Figure 2.

a. Experimental schematic for reversion of hESCs from the primed to naive state. b. Quantification of CD75 positive, CD24 negative (naive) populations in the KLF4 and TFCP2L1 mutants and controls as evaluated by flow cytometry between D17–20 (n=3 each mutant cell line) c. Representative immunofluorescence image at passage 3 (D17-D19) reversions with KLF4 control and KO subline Scale bars show 50 microns. d. Representative immunofluorescence image at passage 3 (D17-D19) reversions with TFCP2L1 control and KO subline. Scale bars show 20 microns.