Figure 7. Comparisons of glomerular fibrotic responses, as determined by Masson’s trichrome staining, to Ang II-induced hypertension treated with or without losartan between male WT and PT-Agtr1a^−/− mice.

(A) WT control mice. (B) WT mice with Ang II infusion. (C) WT mice with concurrent Ang II infusion and losartan treatment. (D) PT-Agtr1a^−/− control mice. (E) PT-Agtr1a^−/− mice with Ang II infusion. (F) PT-Agtr1a^−/− mice with concurrent Ang II infusion and losartan treatment. Note that Ang II induced marked fibrotic response in the glomerulus (B), compared with control WT mice (A). The glomerular fibrotic response was almost completely prevented by concurrent treatment with losartan (C). In PT-Agtr1a^−/− mice, however, Ang II-induced glomerular injury was markedly attenuated (E vs. B), compared with control PT-Agtr1a^−/− mice (D). Further treatment with losartan revealed no significant further effect in PT-Agtr1a^−/− mice (F). G, glomerulus; PT, proximal tubule. Magnification: ×40.