Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 17;12:861078. doi: 10.3389/fonc.2022.861078

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Tirrò, Massimino, Broggi, Romano, Minasi, Gianno, Antonelli, Motta, Certo, Altieri, Manzella, Caltabiano, Barbagallo, Buttarelli, Magro, Giangaspero and Vigneri

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Detection of mutant IDH1 by immunohistochemistry, NGS and Sanger sequencing. (A) Astrocytoma cells are diffusely and strongly stained with anti-IDH1 (R132H) antibody (immunoperoxidase; original magnification 200x). (B) Corresponding IDH1 c.395G>A (p. R132H) mutation detected by NGS. Results were viewed in the Integrative Genomics Viewer (IGV). (C) The chromatogram showing representative sequencing results of IDH1 c.395G>A (p. R132H). (D) Absence of IDH1 (R132H) immunoreactivity in a IDH1- (R132S)-mutant astrocytoma. (E) IDH1 c.394C>A (p. R132S) mutation detected by NGS. Results were viewed in the IGV. (F) The chromatogram showing the representative sequencing results of IDH1 c.394C>A. Note that IDH1 is a negative-sense gene with respect to the genomic reference sequence. Thus, any nucleotide change is displayed as reverse complement. The arrow symbols on chromatogram indicate the place of mutation.