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. 2022 Mar 17;13:813272. doi: 10.3389/fphar.2022.813272

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Copyright © 2022 Liu, Xu, Zang, Lou, Hang, Gao, Shi, Liu, Wang, Sun, Liu, Zhang, Liu and Dong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HL inhibited the remodelling of metabolic factors and acetylation level of key metabolic enzyme in rabbit of AF model. (A) Representative bands of protein expression of Sirt3, AceCS2, Ac-LACD and Ac-AceCS2. (B,C,D,E,F) Quantification analyzing of protein expression of Sirt3, AceCS2, Ac-LACD and Ac-AceCS2. (G and H) Statistical results for ratio of Ac-LCAD/LCAD and Ac-AceCS2/AceCS2. (I) Statistical results for expression of Sirt3 gene.(K) Statistical results for expression of LCAD, CROT, PGC1α,PDH,PDK4, LDHa, AceCS2, NDUFA9, SDHa and GDH genes. Data from these proteins were normalized to β-actin, and genes were normalized to β-actin. *p < 0.05 vs. sham group, **p < 0.01 vs. sham group, ***p < 0.001 vs. sham group; #p < 0.05 vs. P group, ##p < 0.01 vs. P group, n = 6 each group.