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The acetylation level of key metabolic enzymes controlled by Sirt3 pathway in vitro. (A) Representative bands for the protein expression of Sirt3, GDH, Ac-GDH, LCAD, Ac-LCAD, pAMPK, AMPK in the HL-1 cells of all groups. (B,C,E,I,J) Statistical analysis of Sirt3, GDH, LCAD, pAMPK1α and AMPK1α expression. (D,F,G,H) Statistical analysis of Ac-GDH, Ac-LCAD, Ac-GDH/GDH, Ac-LCAD/LCAD. *p < 0.05, **p < 0.01, n = 3 each group.
